I. Foundational Principles of TRIzol Chemistry TRIzol reagent employs a triphasic chemical system to isolate intact RNA through sequential dissociation of cellular components: Cellular Disruption: Guanidinium thiocyanate denatures proteins and inactivates RNases Acidic phenol lyses cells while maint … TRIzol RNA Extraction: Principles, Protocols, and Precision PracticesRead more