I. Clinical Presentation and Diagnostic Dilemma A 57-year-old immunocompromised patient (post-hematopoietic stem cell transplant) presented with persistent fever and respiratory distress lasting 14 days. Initial RT-PCR detected influenza A RNA in bronchoalveolar lavage (BAL) fluid but failed to dist … Case Study: Strand-Specific Identification of Influenza A Virus Replication in an Immunocompromised PatientRead more
RnaMod
Experimental Strategies for Distinguishing Positive-Sense and Negative-Sense RNA Viruses
I. Molecular Signatures Guiding Experimental Design Positive-sense RNA (+ssRNA) and negative-sense RNA (-ssRNA) viruses exhibit fundamental mechanistic differences exploitable for laboratory identification: +ssRNA Viruses: Genomes function as immediate mRNA and produce replicative dsRNA intermediate … Experimental Strategies for Distinguishing Positive-Sense and Negative-Sense RNA VirusesRead more
Distinguishing Positive-Sense and Negative-Sense RNA Viruses: Molecular Mechanisms and Clinical Implications
I. Genomic Identity and Functional Dichotomy Positive-Sense RNA Viruses (+ssRNA) Direct mRNA Function: The +ssRNA genome acts as immediate messenger RNA upon host cell entry. Its sequence is directly recognized by host ribosomes, enabling instantaneous protein synthesis without prior transcription . … Distinguishing Positive-Sense and Negative-Sense RNA Viruses: Molecular Mechanisms and Clinical ImplicationsRead more
Positive-Sense vs. Negative-Sense RNA Viruses: Molecular Mechanisms and Clinical Implications
I. Genomic Identity and Translation Capacity Positive-Sense RNA Viruses (+ssRNA) Genome as mRNA: The +ssRNA genome functions as immediate messenger RNA upon host cell entry, with its nucleotide sequence directly recognized by host ribosomes for instantaneous protein synthesis . Infectious RNA: Purif … Positive-Sense vs. Negative-Sense RNA Viruses: Molecular Mechanisms and Clinical ImplicationsRead more
Mechanism of Negative-Strand RNA Synthesis Directed by Positive-Sense RNA Genomes
I. Molecular Framework of Replication Initiation Positive-sense RNA (+ssRNA) viruses orchestrate negative-strand RNA synthesis through a precisely regulated sequence of events: Genome Translation: Upon host cell entry, +ssRNA acts as mRNA for immediate synthesis of RNA-dependent RNA polymerase (RdRp … Mechanism of Negative-Strand RNA Synthesis Directed by Positive-Sense RNA GenomesRead more
Mechanisms of Negative-Strand RNA Synthesis Mediated by Positive-Sense RNA Genomes
I. Molecular Framework of Replication Initiation Positive-sense RNA (+ssRNA) genomes serve dual roles upon host cell entry: Immediate Translation Template: Direct synthesis of viral replicase complexes (RdRp, helicases, cofactors) Replication Blueprint: Formation of membrane-bound replication organe … Mechanisms of Negative-Strand RNA Synthesis Mediated by Positive-Sense RNA GenomesRead more
Negative-Strand RNA Transcription: Molecular Mechanisms of mRNA Synthesis
I. Genomic Constraints and Primer Requirements Negative-sense RNA viruses (-ssRNA) possess genomes that are functionally inert upon host cell entry: Non-translatable Sequence: The genome is complementary to mRNA, preventing direct ribosome binding RdRp Dependency: Virion-packaged RNA-dependent RNA p … Negative-Strand RNA Transcription: Molecular Mechanisms of mRNA SynthesisRead more
The Expanding Frontier: RNA Probes Revolutionizing Biomedical Applications
I. Clinical Diagnostics: Precision at Molecular Scale RNA probes enable transformative disease detection by targeting pathogen genomes and cancer biomarkers with single-molecule resolution: Infectious Disease Panels Multiplexed respiratory virus detection (SARS-CoV-2, influenza, RSV) via microfluidi … The Expanding Frontier: RNA Probes Revolutionizing Biomedical ApplicationsRead more