viral

I. Clinical Presentation and Diagnostic Dilemma A 57-year-old immunocompromised patient (post-hematopoietic stem cell transplant) presented with persistent fever and respiratory distress lasting 14 days. Initial RT-PCR detected influenza A RNA in bronchoalveolar lavage (BAL) fluid but failed to dist … Case Study: Strand-Specific Identification of Influenza A Virus Replication in an Immunocompromised PatientRead more

I. Molecular Signatures Guiding Experimental Design Positive-sense RNA (+ssRNA) and negative-sense RNA (-ssRNA) viruses exhibit fundamental mechanistic differences exploitable for laboratory identification: +ssRNA Viruses: Genomes function as immediate mRNA and produce replicative dsRNA intermediate … Experimental Strategies for Distinguishing Positive-Sense and Negative-Sense RNA VirusesRead more

I. Genomic Identity and Functional Dichotomy Positive-Sense RNA Viruses (+ssRNA) Direct mRNA Function: The +ssRNA genome acts as immediate messenger RNA upon host cell entry. Its sequence is directly recognized by host ribosomes, enabling instantaneous protein synthesis without prior transcription . … Distinguishing Positive-Sense and Negative-Sense RNA Viruses: Molecular Mechanisms and Clinical ImplicationsRead more

I. Genomic Identity and Translation Capacity Positive-Sense RNA Viruses (+ssRNA) Genome as mRNA: The +ssRNA genome functions as immediate messenger RNA upon host cell entry, with its nucleotide sequence directly recognized by host ribosomes for instantaneous protein synthesis . Infectious RNA: Purif … Positive-Sense vs. Negative-Sense RNA Viruses: Molecular Mechanisms and Clinical ImplicationsRead more