CRISPR + Single-Cell Sequencing: A Deep Dive into Technical Integration 1. Definition and Core Principles CRISPR + Single-Cell Sequencing is an innovative approach that combines CRISPR gene-editing technology with single-cell resolution omics analysis (e.g., single-cell RNA sequencing). Its goal is … CRISPR + Single-Cell Sequencing: A Deep Dive into Technical IntegrationRead more
CRISPR
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a revolutionary gene-editing technology derived from a natural bacterial immune system.
Key points:
Uses a guide RNA to direct the Cas9 protein to cut specific DNA sequences.
Allows precise insertion, deletion, or modification of genes.
Applications: curing genetic diseases, improving crops, biomedical research.
Nobel Prize in Chemistry (2020) awarded to Doudna & Charpentier for its discovery.
It’s faster, cheaper, and more accurate than older gene-editing methods.
CRISPR-Toolkit: Definition, Core Components, and Applications
CRISPR-Toolkit: Definition, Core Components, and Applications Definition and Conceptual Scope CRISPR-Toolkit refers to a collection of software, experimental reagents, and standardized protocols developed around CRISPR gene-editing technology. It aims to simplify the complexity of genetic editing wh … CRISPR-Toolkit: Definition, Core Components, and ApplicationsRead more
Neurodecode: Definition, Technical Insights, and Applications
1. Terminology and Core Concepts Neurodecode is a compound term with two primary meanings: Neural Decoding (General Concept): The process of extracting information related to external stimuli, behaviors, or cognitive states from neural activity signals (e.g., EEG, fMRI, single-neuron recordings). It … Neurodecode: Definition, Technical Insights, and ApplicationsRead more
Haptisense: Definition, Mechanisms, and Cross-Disciplinary Applications
Etymology and Core Definition Haptisense (or haptic sense) originates from the Greek haptikos (“touchable”) and Latin sensus (“perception”), translating to a tactile perception system. It is defined as: A biological system that integrates mechanical stimuli (e.g., pressure, v … Haptisense: Definition, Mechanisms, and Cross-Disciplinary ApplicationsRead more
CRISPR Target: Definition, Mechanisms, and Applications
1. Definition and Core Mechanisms CRISPR Target refers to the DNA or RNA sequences specifically recognized and cleaved by CRISPR systems (e.g., Cas9, Cas13 nucleases) guided by RNA (gRNA or crRNA). This process underpins CRISPR’s ability to achieve gene editing, antiviral immunity, or diagnostic fun … CRISPR Target: Definition, Mechanisms, and ApplicationsRead more
ECOSynBio:Energy and Carbon Optimized Synthesis for the Bioeconomy
ECOSynBio (Energy and Carbon Optimized Synthesis for the Bioeconomy) is an innovative initiative launched in 2020 by the U.S. Department of Energy’s Advanced Research Projects Agency-Energy (ARPA-E). It aims to redefine biomanufacturing through synthetic biology and bioengineering to achieve efficie … ECOSynBio:Energy and Carbon Optimized Synthesis for the BioeconomyRead more